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Carbon Nanotube Translocation to Distant Organs after Pulmonary Exposure: Insights from in Situ 14C-Radiolabeling and Tissue Radioimaging
Link to Journal Abstract
Few approaches are available to investigate the potential of carbon nanotubes (CNTs) to translocate to distant organs following lung exposure, although this needs to be taken into account to evaluate potential CNT toxicity. Here, we report a method for quantitative analysis of the tissue biodistribution of multiwalled CNTs (MWCNTs) as a function of time. The method relies on the use of in situ 14C-radiolabeled MWCNTs and combines radioimaging of organ tissue sections to ex vivo analysis of MWCNTs by electron microscopy. To illustrate the usefulness of this approach, mice were exposed to a single dose of 20 ėg of 14C-labeled MWCNTs by pharyngeal aspiration and were subjected to a follow-up study over one year. After administration, MWCNT were cleared from the lungs, but there was a concomitant relocation of these nanoparticles to distant organs starting throughout the follow-up period, with nanoparticle accumulation increasing with time. After one year, accumulation of MWCNTs was documented in several organs, including notably the white pulp of the spleen and the bone marrow. This study shows that the proposed method may be useful to complement other approaches to address unresolved toxicological issues associated with CNTs. These issues include their persistence over long periods in extrapulmonary organs, the relationship between the dose and the extent of translocation, and the effects of safety by design on those processes. The same approach could be used to study the translocation propensity of other nanoparticles containing carbon atoms.
In this paper, the authors report a method for quantitative analysis of the tissue biodistribution of multiwalled carbon nanotubes (MWCNTs) as a function of time. The method relies on the use of in situ 14C-radiolabeled MWCNTs and combines radioimaging of organ tissue sections to ex vivo analysis of MWCNTs by electron microscopy. To illustrate the usefulness of this approach, mice were exposed to a single dose of 20 ėg of 14C-labeled MWCNTs by pharyngeal aspiration and were subjected to a follow-up study over one year.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Material Analysis and Applications
Risk Exposure Group
ACS Nano, 2014, 8(6): 5715-5724
Czarny B, Georgin D, Berthon F, Plastow G, Pinault M, Patriarche G, Thuleau A, L'Hermite MM, Taran F, Dive V
Last updated on July 4, 2014
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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