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In vitro toxicological assessment of clays for their use in food packaging applications
Link to Journal Abstract
Montmorillonite based clays have a wide range of applications that are going to contribute to increase human exposure to these materials. One of the most promising uses of clays is the development of reinforced food contact materials that results in nanocomposites with improved barrier properties. Different organoclays have been developed introducing modifiers in the natural clay which is commercially available. However, the toxicological aspects of these materials have been scarcely studied so far. In the present study, the cytotoxic effects of a non-modified clay (CloisiteŽ Na+) and an organoclay (CloisiteŽ 30B) have been investigated in the hepatic cell line HepG2. Only CloisiteŽ 30B showed cytotoxicity. In order to elucidate the toxic mechanisms underlying these effects, apoptosis, inflammation, oxidative stress and genotoxicity biomarkers were assayed. Moreover, a morphology study with light and electron microscopy was performed. Results showed genotoxic effects and glutathione decrease. The most relevant ultraestructural alterations observed were mitochondrial degeneration, dilated endomembrane systems, heterophagosomes formation, fat droplets appearance and presence of nuclear lipid inclusions. CloisiteŽ 30B, therefore, induces toxic effects in HepG2 cells. Further research is needed to assess the risk of this clay on the human health.
In the present study, the cytotoxic effects of a non-modified clay (CloisiteŽ Na+) and an organoclay (CloisiteŽ 30B) have been investigated in the hepatic cell line HepG2. In order to elucidate the toxic mechanisms underlying these effects, apoptosis, inflammation, oxidative stress and genotoxicity biomarkers were assayed.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Food and Chemical Toxicology, 57: 266-275 (July 2013)
Food and Chemical Toxicology
Maisanaba S, Puerto M, Pichardo S, Jorda M, Moreno FJ, Aucejo S, Jos A
Last updated on August 14, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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