ICON Web & News
Search Using OECD Database
Return to Previous Page
Addition or Correction
Role of carbonaceous nanomaterials in stimulating osteogenesis in mammalian bone cells
Link to Journal Abstract
In this work, we demonstrate that graphitic nanomaterials - carboxylated multi-walled carbon nanotubes (MWCNTs) and carboxylated graphenes (Gn) - have the ability to stimulate the process of osteogenesis in mammalian bone cells and significantly increase the level of bone mineralization. Exposure of MC3T3-E1 bone cells to carboxylated MWCNTs - nano-sized (nano-Gn) and micro-sized (micro-Gn) in concentrations of 1-10 ėg ml-1 - resulted in the enhancement of mineralization in a time-dependent manner for the cells exposed to the nanomaterials, as compared to unexposed cells. However, the graphitic nanomaterials did not show significant toxicity in the concentration levels that were studied. Gene expression analysis revealed that the MWCNTs activated expression of the mid-stage osteogenic marker, Col I, on the 12th day of cell incubation. The gene expression of the earliest osteogenic marker, Cbfa-1, and the downstream effector of BMP signaling, SMAD1, were significantly increased in bone cells exposed to both materials (MWCNTs and nano-Gn) as compared to unexposed control cells. Our data clearly demonstrate the ability of graphitic nano-materials to penetrate bone cells and regulate deposition of minerals in an in vitro model system. Our findings highlight the potential use of such materials in regenerative nanomedicine.
In this work, the authors investigate whether graphitic nanomaterials - carboxylated multi-walled carbon nanotubes (MWCNTs) and carboxylated graphenes (Gn) - have the ability to stimulate the process of osteogenesis in mammalian bone cells and how the level of bone mineralization is affected.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Journal of Materials Chemistry B, 2013, 1(25): 3220-3230
Journal of Materials Chemistry B
Mahmood M, Villagarcia H, Dervishi E, Mustafa T, Alimohammadi M, Casciano D, Khodakovskaya M, Biris AS
Last updated on August 5, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
Why Join Us?
Mission and Strategy
Good Nano Guide
Nano EHS Research Needs
Current Practices Survey