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Cytotoxicity of cuprous oxide nanoparticles to fish blood cells: hemolysis and internalization
Link to Journal Abstract
Cuprous oxide nanoparticles (Cu2O NPs) possess unique physical and chemical properties which are employed in a broad variety of applications. However, little is known about the adverse effects of Cu2O NPs on organisms. In the current study, in vitro cytotoxicity of Cu2O NPs (ca. 60 nm in diameter) to the blood cells of freshwater fish Carassius auratus was evaluated. A concentration-dependent hemolytic activity of Cu2O NPs to red blood cells (RBCs) and the phagocytosis of Cu2O NPs by leukocytes were revealed. The results showed that dosages of Cu2O NPs greater than 40 ėg/mL were toxic to blood cells, and could cause serious membrane damage to RBCs. The EC50 value of Cu2O NPs as obtained from RBCs and whole blood exposure was 26 and 63 ėg/mL, respectively. The generation of reactive oxygen species and the direct interaction between Cu2O NPs and the cell membrane were suggested as the possible mechanism for cytotoxicity, and the intrinsic hemolytic active of Cu2O NPs was the main contributor to the toxicity rather than solubilized copper ions. The adsorption of plasma proteins on the surfaces of Cu2O NPs led to their aggregation in whole blood, and aggregate formation can significantly alleviate the hemolytic effect and subsequently mediate the phagocytosis of Cu2O NPs by leukocytes.
In this study, in vitro cytotoxicity of cuprous oxide nanoparticles (Cu2O NPs), about 60 nm in diameter, to the blood cells of freshwater fish Carassius auratus was evaluated.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Environmental Fate and Transport
Risk Exposure Group
Journal of Nanoparticle Research, 2013, 15(3): 1507
Journal of Nanoparticle Research
Chen LQ, Kang B, Ling J
Last updated on June 21, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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