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Method for toxicity test of titanium dioxide nanoparticles in ciliate protozoan Tetrahymena
Link to Journal Abstract
Titanium dioxide (TiO2) nanoparticles have a high surface-to-mass ratio and rapidly aggregate in water causing great difficulties for toxicity test exposed to aquatic organisms or other cell lines. This study uses a cell viability kit for routine toxicity test of TiO2 as well as other nanoparticles which accumulate in the aquatic environment. Tetrahymena immediately endocytoses TiO2 nanoparticles and stores them in food vacuoles until the particles undergo exocytosis as larger aggregates. However, during the process of endocytosis and exocytosis, TiO2 particles interfere with cell growth and consequently induce acute toxicity. It exerted high cell growth inhibition at 20 h incubation and induces significant cytotoxic effects. Surprisingly, the effect of TiO2 decreases at 40 h incubation, due to the recovery of cell growth and reduction of the cytotoxicity of the particles.
This study uses a cell viability kit for routine toxicity test of titanium dioxide (TiO2) nanoparticles as well as other nanoparticles which accumulate in the aquatic environment.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Environmental Fate and Transport
Risk Exposure Group
Journal of Environmental Science and Health, Part A, 2013, 48(11): 1343-1348
Journal of Environmental Science and Health, Part A
Ud-Daula A, Pfister G, Schramm KW
Last updated on June 14, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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