International Council on Nanotechnology

ICON Web & News Virtual Journal Everything

Resources
	Virtual Journal
	Analyze Database
	GoodNanoGuide
	ICON Reports
	ICON Backgrounders
	Glossary
	Policy Reports
	Ratings Comment Guidelines
	Links

Quick Search:
Keywords: Search: Search Using OECD Database

Details

Return to Previous Page

Addition or Correction

Title:

Stability of Nanoparticle Suspensions in Different Biologically Relevant Media

Date:

12/2012

Link to Journal Paper

Abstract:

For application of nanoparticles (NPs) as delivery vectors for biomedical applications the size, charge, surface chemistry and aggregation of the particles are one of key parameters. We focused on characterization of maghemite and cobalt ferrite magnetic NPs by measuring size distribution and zeta potential in different culture media and PBS. We show that our NPs functionalized with PAA are relatively very stable also in different culture media, where level of aggregation depends on medium composition. Effect of divalent ions and serum presence on stability is specifically analysed and we present possible destabilization mechanisms. We show that stability of electrostatically stabilized suspensions is affected by the molar concentration and valence of destabilizing counterions like Na+ or Mg2+ where effective surface charge of nanoparticles and thus repulsion force is screened by these counterions. In agreement with other papers we demonstrate significant effect of media composition (divalent ions, serum) and thus show the importance of NPs characterization under conditions that are representative of cell culture media or physiological conditions for understanding of NPs interaction with biological systems and for assessments of nanoparticle cytotoxicity. Further, by understanding the destabilization mechanisms one can anticipate effect in different media and to some degree predict behaviour of nanoparticle suspensions.

Non-technical Summary:

This study focuses on characterization of maghemite and cobalt ferrite magnetic nanoparticles (NPs) by measuring size distribution and zeta potential in different culture media and PBS. The effect of media composition (divalent ions, serum), which can show the importance of NPs characterization under conditions that are representative of cell culture media or physiological conditions for understanding of NPs interaction with biological systems and for assessments of nanoparticle cytotoxicity was also evaluated.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Other/Unspecified

Exposure Pathway

Other/Unspecified

Method Of Study

Material Analysis and Applications

Paper Type

Applications

Particle Type

Other/Unspecified