International Council on Nanotechnology

ICON Web & News Virtual Journal Everything

Resources
	Virtual Journal
	Analyze Database
	GoodNanoGuide
	ICON Reports
	ICON Backgrounders
	Glossary
	Policy Reports
	Ratings Comment Guidelines
	Links

Quick Search:
Keywords: Search: Search Using OECD Database

Details

Return to Previous Page

Addition or Correction

Title:

Visualization of custom-tailored iron oxide nanoparticles chemistry, uptake, and toxicity

Date:

12/2012

Link to Journal Abstract

Abstract:

Nanoparticles of iron oxide generated by wearing of vehicles have been modelled with a tailored solution of size-uniform engineered magnetite particles produced by the Bradley reaction, a solvothermal metal�organic approach rendering hydrophilic particles. The latter does not bear any pronounced surface charge in analogy with that originating from anthropogenic sources in the environment. Physicochemical properties of the nanoparticles were thoroughly characterized by a wide range of methods, including XPD, TEM, SEM, DLS and spectroscopic techniques. The magnetite nanoparticles were found to be sensitive for transformation into maghemite under ambient conditions. This process was clearly revealed by Raman spectroscopy for high surface energy magnetite particles containing minor impurities of the hydromaghemite phase and was followed by quantitative measurements with EXAFS spectroscopy. In order to assess the toxicological effects of the produced nanoparticles in humans, with and without surface modification with ATP (a model of bio-corona formed in alveolar liquid), a pathway of potential uptake and clearance was modelled with a sequence of in vitro studies using A549 lung epithelial cells, lymphocyte 221-B cells, and 293T embryonal kidney cells, respectively. Raman microscopy unambiguously showed that magnetite nanoparticles are internalized within the A549 cells after 24 h co-incubation, and that the ATP ligand is retained on the nanoparticles throughout the uptake process. The toxicity of the nanoparticles was estimated using confocal fluorescence microscopy and indicated no principal difference for unmodified and modified particles, but revealed considerably different biochemical responses. The IL-8 cytokine response was found to be significantly lower for the magnetite nanoparticles compared to TiO2, while an enhancement of ROS was observed, which was further increased for the ATP-modified nanoparticles, implicating involvement of the ATP signalling pathway in the epithelium.

Non-technical Summary:

In this study, nanoparticles of iron oxide generated by wearing of vehicles have been modelled with a tailored solution of size-uniform engineered magnetite particles produced by the Bradley reaction. Physicochemical properties of the nanoparticles were thoroughly characterized and the toxicological effects of the produced nanoparticles were assessed in humans, with and without surface modification with ATP (a model of bio-corona formed in alveolar liquid), a pathway of potential uptake and clearance was modelled with a sequence of in vitro studies using A549 lung epithelial cells, lymphocyte 221-B cells, and 293T embryonal kidney cells, respectively.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Mammalian

Exposure Pathway

Other/Unspecified

Method Of Study

Computational and System Modeling

Paper Type

Particle Type

Production Method

Risk Exposure Group

Target Audience

Citation:

Nanoscale, 2012, 4(23): 7383-7393

Publication:

Nanoscale

Author:

Wilkinson K, Ekstrand-Hammarstrom B, Ahlinder L, Guldevall K, Pazik R, Kepinski L, Kvashnina KO, Butorin SM, Brismar H, Onfelt B, Osterlund L, Seisenbaeva GA, Kessler VG

Volume:

Number:

Pages:

7383-7393

Last updated on January 8, 2013

Permalink

| | | | | | | |

Why Join Us?

Mission and Strategy

Background

Organization

Quick Search: