International Council on Nanotechnology

ICON Web & News Virtual Journal Everything

Resources
	Virtual Journal
	Analyze Database
	GoodNanoGuide
	ICON Reports
	ICON Backgrounders
	Glossary
	Policy Reports
	Ratings Comment Guidelines
	Links

Quick Search:
Keywords: Search: Search Using OECD Database

Details

Return to Previous Page

Addition or Correction

Title:

Nano copper induced apoptosis in podocytes via increasing oxidative stress

Date:

11/2012

Link to Journal Abstract

Abstract:

Nanosized copper particles (nano-Cu), one of the representative metal nanometer materials, were used in several domains, and the potential toxicity was raised more and more attention. In order to investigate the cytotoxicity induced by nano-Cu in podocytes, which was the key player of the glomerular filtration barrier, podocytes were treated with different concentrations of nano-Cu. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the cell viability. Hoechst 33342 staining assay and Annexin V/PI double labeling assay were used to identify whether the cytotoxicity induced by nano-Cu was due to apoptosis or necrosis. The oxidative stress induced by nano-Cu and its mechanism were studied in relation to the generation of reactive oxygen species (ROS), superoxide dismutase (SOD) and malondialdehyde (MDA). As a result, while podocytes were treated with nano-Cu, the cell viability was significantly decreased and the apoptosis was significantly increased in podocytes. Results showed that nano-Cu affected the oxidant-antioxidant balance and had cytotoxicity in podocytes, resulting in the enhanced generation of ROS and MDA. Meanwhile, pretreatment with N-(2-mercaptopropionyl)-glycine (N-MPG), a type of ROS scavenger, could inhibit podocyte apoptosis induced by nano-Cu. Results suggested that the increased oxidative stress was a key mechanism in the podocyte apoptosis induced by nano-Cu, which could provide evidence for further research on the toxicity of nano-Cu.

Non-technical Summary:

In order to investigate the cytotoxicity induced by nano-Cu in podocytes, which was the key player of the glomerular filtration barrier, podocytes were treated with different concentrations of nano-Cu for this study. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to measure the cell viability.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Exposure Pathway

Method Of Study

Paper Type

Particle Type