International Council on Nanotechnology

ICON Web & News Virtual Journal Everything

Resources
	Virtual Journal
	Analyze Database
	GoodNanoGuide
	ICON Reports
	ICON Backgrounders
	Glossary
	Policy Reports
	Ratings Comment Guidelines
	Links

Quick Search:
Keywords: Search: Search Using OECD Database

Details

Return to Previous Page

Addition or Correction

Title:

In Vitro Cytotoxicity Assay on Gold Nanoparticles with Different Stabilizing Agents

Date:

7/2012

Link to Journal Abstract

Abstract:

Noble gold nanoparticles (AuNps) are generally nontoxic due to their inert nature. The gold nanoparticles are easily tagged with various proteins and biomolecules rich in aminoacid leading to important biomedical applications including targeted drug delivery, cellular imaging, and biosensing. In this study, three cytotoxicity detection assays 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red cell, and lactate dehydrogenase (LDH) on gold nanoparticles stabilized with citrate, starch, and gum arabic are used. The assays used are based on different mode of detection like LDH release, MTT metabolism, and neutral red uptake. We found that the AuNps stabilized with citrate are very sensitive to the change of concentration and time assay compared to starch and gum arabic stabilized gold nanoparticles.

Non-technical Summary:

In this study, three cytotoxicity detection assays 3-(4,5-dimethylthiozol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), neutral red cell, and lactate dehydrogenase (LDH) on gold nanoparticles stabilized with citrate, starch, and gum arabic are used. The assays used are based on different mode of detection like LDH release, MTT metabolism, and neutral red uptake.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Exposure Pathway

Method Of Study

Paper Type

Particle Type