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Toxicity of iron oxide nanoparticles against osteoblasts
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Magnetic nanoparticles have been widely used for tissue repair, magnetic resonance imaging, immunoassays and drug delivery. They are very promising in orthopaedic applications and several magnetic nanoparticles have been exploited for the treatment of orthopaedic disease. Here, we conducted an in vitro study to examine the interaction of magnetic iron oxide nanoparticles with human osteoblasts to evaluate the dose-related toxicity of the nanoparticles on osteoblasts. A transmission electron microscope was used to visualise the internalised magnetic nanoparticles in osteoblasts. The CCK-8 results revealed increased cell viability (107.5 % vitality compared with the control group) when co-cultured at a low concentration (20 ?g/mL) and decreased cell viability (59.5 % vitality in a concentration of 300 ?g/mL and 25.9 % in 500 ?g/mL) when co-cultured in high concentrations. The flow cytometric detection revealed similar results with 5.48 % of apoptosis in a concentration of 20 ?g/mL, 23.40 % of apoptosis in a concentration of 300 ?g/mL and 28.49 % in a concentration of 500 ?g/mL. The disrupted cytoskeleton of osteoblasts was also revealed using a laser scanning confocal microscope. We concluded that use of a low concentration of magnetic iron oxide nanoparticles is important to avoid damage to osteoblasts.
In this study the authors conducted an in vitro study to examine the interaction of magnetic iron oxide nanoparticles with human osteoblasts to evaluate the dose-related toxicity of the nanoparticles on osteoblasts.
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Journal of Nanoparticle Research, 14(9): 1091 (September 2012)
Journal of Nanoparticle Research
Shi SF, Jia JF, Guo XK, Zhao YP, Liu BY, Chen DS, Guo YY, Zhang XL
Last updated on October 19, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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