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Title: Effects of copper nanoparticles on rat cerebral microvessel endothelial cells
Date: 6/2012
  Link to Journal Abstract
Abstract: The purpose of the current study was to determine whether copper nanoparticles (Cu-NPs) can induce the release of proinflammatory mediators that influence the restrictive characteristics of the blood–brain barrier. Material & methods: Confluent rat brain microvessel endothelial cells (rBMECs) were treated with well-characterized Cu-NPs (40 or 60 nm). Cytotoxicity of the Cu-NPs was evaluated by cell proliferation assay (1.5–50 µg/ml). The extracellular concentrations of proinflammatory mediators (IL-1â, IL-2, TNF-á and prostaglandin E2) were evaluated by ELISA. Results: The exposure of Cu-NPs at low concentrations increases cellular proliferation of rBMECs, by contrast, high concentrations induce toxicity. Prostaglandin E2 release was significantly increased (threefold; 8 h) for Cu-NPs (40 and 60 nm). The extracellular levels of both TNF-á and IL-1â were significantly elevated following exposure to Cu-NPs. The P-apparent ratio, as an indicator of increased permeability of rBMEC was approximately twofold for Cu-NPs (40 and 60 nm). Conclusion: These data suggest that Cu-NPs can induce rBMEC, proliferation at low concentrations and/or induce blood–brain barrier toxicity and potential neurotoxicity at high concentrations.
Non-technical Summary: The purpose of the current study was to determine whether copper nanoparticles (Cu-NPs) can induce the release of proinflammatory mediators that influence the restrictive characteristics of the blood–brain barrier. Confluent rat brain microvessel endothelial cells (rBMECs) were treated with well-characterized Cu-NPs (40 or 60 nm). Cytotoxicity of the Cu-NPs was evaluated by cell proliferation assay (1.5–50 µg/ml). The extracellular concentrations of proinflammatory mediators (IL-1â, IL-2, TNF-á and prostaglandin E2) were evaluated by ELISA.
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Mammalian
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Metal
Production Method Engineered
Risk Exposure Group General Population
Target Audience Technical Research
Citation: Nanomedicine, 7(6): 835-846 (June 2012)
Publication: Nanomedicine
Author: Trickler WJ, Lantz SM, Schrand A, Robinson BL, Newport GD, Schlager JJ, Paule MG, Slikker W, Biris AS, Hussain SM, Ali SF
Volume: 7
Number: 6
Pages: 835-846
     


Last updated on July 11, 2012
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
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