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Effect of particle size on in vitro cytotoxicity of titania and alumina nanoparticles
Link to Journal Abstract
Aluminium oxide (Al2O3) and titanium dioxide (TiO2) nanoparticles (NPs) have been widely used in nanotechnology-based products. Recently, researchers and the public have raised concerns about the adverse effects of these NPs in biological systems, particularly in humans. The aim of this study was to investigate the possible adverse effects of these two common metal oxide NPs on human lung epithelium cells (A549) and to investigate NP size-dependent effects on these cells, considering both the primary and hydrodynamic particle size. NPs were found to inhibit cell viability and proliferation at the highest concentration level (10 mg/mL) included in this study, as measured by a clonogenic assay. Moreover, cell viability, proliferation and metabolism were impaired to a greater extent by the smaller NPs (5 nm TiO2 and 10 nm Al2O3) relative to the larger particles (200 nm TiO2 and 50 nm Al2O3) included in this study, as measured by cell proliferation and metabolism. Notably, the observed cytotoxic effects correlated to the primary size, rather than the hydrodynamic size. Similarly, NP cytotoxicity was found to be correlated with the NP surface area. These findings highlight the importance of including primary size and surface area information in NP characterisation in cytotoxicity studies.
The aim of this study was to investigate the possible adverse effects of two common metal oxide nanoparticles (NPs), aluminum oxide (Al2O3) and titanium oxice (TiO2), on human lung epithelium cells (A549) and to investigate NP size-dependent effects on these cells, considering both the primary and hydrodynamic particle size.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Journal of Experimental Nanoscience, 2014, 9(6): 625-638
Journal of Experimental Nanoscience
Wei Z, Chen L, Thompson DM, Montoya LD
Last updated on July 2, 2014
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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