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Evaluation of Silver Nanoparticle Toxicity to RAW264.7 Cells in a Three-Dimensional Cell Culture
Link to Journal Abstract
Clinically relevant in vitro models are required to rapidly evaluate the cytotoxicity of nanoparticles. Recently, numerous three-dimensional cell culture systems (3D) have been manufactured to mimic the properties of tissue in vivo. Spheroid culture models play an invaluable role in understanding tumor biology and in performing the screening of drugs and biomaterials. Nanoparticles (NPs) are currently receiving considerable attention for industrial and biomedical applications, yet their hazardous and potential toxic effects have not been studied extensively. The purpose of this study was to evaluate the biological effects of polyvinylpyrrolidone-coated silver nanoparticles (AgNPs) in murine macrophages (RAW264.7 cells) cultured in traditional two-dimensional (2D) and novel 3D systems. Biological assays and a micromorphological analysis was performed. Microscopic imaging revealed that the particles were internalized into cells and receptor-mediated endocytosis occurred. However, cells exposed to AgNPs exhibited minor morphological changes compared with cells no exposed. AgNPs displayed greater cytotoxicity in the 2D culture versus in the 3D. Moreover, in the 3D culture the AgNP cytotoxicity decreased over time, which did not occur in the 2D culture.
The purpose of this study was to evaluate the biological effects of polyvinylpyrrolidone-coated silver nanoparticles (AgNPs) in murine macrophages (RAW264.7 cells) cultured in traditional two-dimensional (2D) and novel 3D systems.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
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Journal of Biomaterials and Tissue Engineering, 4(1): 51-58 (January 2014)
Journal of Biomaterials and Tissue Engineering
Hashimoto M, Toshima H, Yonezawa T, Kawai K, Iijima M, Endo K
Last updated on January 28, 2014
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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