ICON Web & News
Search Using OECD Database
Return to Previous Page
Addition or Correction
Silver-titanium dioxide nanocomposites as effective antimicrobial and antibiofilm agents
Link to Journal Abstract
Ag–TiO2 nanocomposites were successfully developed from colloidal suspensions containing 750 or 1,500 ppm silver nanoparticles (AgNPs) deposited on 5 % (w/v) titanium dioxide nanoparticles (TiO2NPs) by a chemical reduction approach. The nanocomposites were characterized by diffuse reflectance UV–Vis spectroscopy (DRS), transmission electron microscopy (TEM), scanning electron microscopy (SEM) with energy-dispersive X-ray spectroscopy (EDX) and dynamic light scattering (DLS). DRS spectra showed an absorption band in visible region with maximum absorbance peaks at 452 and 444 nm attributed to AgNPs plasmon peaks, indicating the formation of small spherical or quasi-spherical Ag nanocrystals in nanocomposites. TEM and SEM analysis proved a nearly spherical morphology of particles (15–30 ± 5 nm average size in diameter). EDX analysis revealed the presence of Ti, O, and Ag in both nanocomposite powders having 1.37 or 2.34 wt% Ag content. DLS analysis yielded a bimodal particle size distribution in a narrow range (31.3 ± 0.5 or 23.4 ± 0.4 nm average particle diameter) and a good polydispersity (0.247 or 0.293 polydispersity index). The nanocomposites were screened for their in vitro antimicrobial activity against Gram-positive (Bacillus subtilis and Staphylococcus aureus) and Gram-negative (Escherichia coli, Klebsiella pneumoniae, and Pseudomonas aeruginosa) bacterial and fungal (Candida albicans) reference and clinical strains, in planktonic and adherent state, by qualitative and quantitative assays. The antibacterial activity increased with the increasing AgNPs content, being more intensive for Gram-positive bacteria. Both Ag–TiO2 nanocomposites exhibited a high antibiofilm activity. The obtained results recommend the use of the developed nanocomposites as antimicrobial and antibiofilm agents in practical applications without UV irradiation. The most effective agent proved to be the one with 2.34 wt% AgNPs content.
This paper describes the preparation of Ag-TiO2 nanocomposites from colloidal suspensions containing 750 or 1,500 ppm silver nanoparticles (AgNPs) deposited on 5 % (w/v) titanium dioxide nanoparticles (TiO2NPs) by a chemical reduction approach. The nanocomposites were characterized by several methods and they were screened for their in vitro antimicrobial activity against Gram-positive and Gram-negative bacterial and fungal reference and clinical strains, in planktonic and adherent state, by qualitative and quantitative assays.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Environmental Fate and Transport
Risk Exposure Group
Journal of Nanoparticle Research, 2013, 16(1): 2203
Journal of Nanoparticle Research
Lungu M, Gavriliu S, Enescu E, Ion I, Bratulescu A, Mihaescu G, Marutescu L, Chifiriuc MC
Last updated on January 23, 2014
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
Why Join Us?
Mission and Strategy
Good Nano Guide
Nano EHS Research Needs
Current Practices Survey