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Effect of Zinc Oxide Nanoparticles on the Function of MC3T3-E1 Osteoblastic Cells
Link to Journal Abstract
Zinc oxide nanoparticles (ZnO NPs) can be ingested directly when used in food, food packaging, drug delivery, and cosmetics. This study evaluated the cellular effects of ZnO NPs (50 and 100 nm diameter particle sizes) on the function of osteoblastic MC3T3-E1 cells. ZnO NPs showed cytotoxicity at concentrations of above 50 レg/ml, and there was no significant effect of the size on the cytotoxicity of ZnO NPs. Within the testing concentrations of 0.01´1 レg/ml, which did not cause a marked drop in cell viability, ZnO NPs (0.1 レg/ml) caused a significant elevation of alkaline phosphatase activity, collagen synthesis, mineralization, and osteocalcin content in the cells (P < 0.05). Moreover, pretreatment with ZnO NPs (0.01´1 レg/ml) significantly reduced antimycin A-induced cell damage by preventing mitochondrial membrane potential dissipation, complex IV inactivation, and ATP loss. Measurement of reactive oxygen species (ROS) indicated decrease in ROS level upon exposure to ZnO nanoparticles (0.01 レg/ml). Hence, our study indicated that ZnO nanoparticles can have protective effects on osteoblasts at low concentrations where there are little or no observable cytotoxic effects.
This study evaluates the cellular effects of zinc oxide nanoparticles (ZnO NPs) (50 and 100 nm diameter particle sizes) on the function of osteoblastic MC3T3-E1 cells
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Biological Trace Element Research, 155(2): 287-294 (November 2013)
Biological Trace Element Research
Suh KS, Lee YS, Seo SH, Kim YS, Choi EM
Last updated on October 14, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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