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Effects of Fe3O4 Magnetic Nanoparticles on A549 Cells
Link to Journal Abstract
Fe3O4 magnetic nanoparticles (MgNPs-Fe3O4) are widely used in medical applications, including magnetic resonance imaging, drug delivery, and in hyperthermia. However, the same properties that aid their utility in the clinic may potentially induce toxicity. Therefore, the purpose of this study was to investigate the cytotoxicity and genotoxicity of MgNPs-Fe3O4 in A549 human lung epithelial cells. MgNPs-Fe3O4 caused cell membrane damage, as assessed by the release of lactate dehydrogenase (LDH), only at a high concentration (100 ėg/mL); a lower concentration (10 ėg/mL) increased the production of reactive oxygen species, increased oxidative damage to DNA, and decreased the level of reduced glutathione. MgNPs-Fe3O4 caused a dose-dependent increase in the CD44+ fraction of A549 cells. MgNPs-Fe3O4 induced the expression of heme oxygenase-1 at a concentration of 1 ėg/mL, and in a dose-dependent manner. Despite these effects, MgNPs-Fe3O4 had minimal effect on cell viability and elicited only a small increase in the number of cells undergoing apoptosis. Together, these data suggest that MgNPs-Fe3O4 exert little or no cytotoxicity until a high exposure level (100 ėg/mL) is reached. This dissociation between elevated indices of cell damage and a small effect on cell viability warrants further study.
The purpose of this study was to investigate the cytotoxicity and genotoxicity of Fe3O4 magnetic nanoparticles (MgNPs-Fe3O4) in A549 human lung epithelial cells.
Peer Reviewed Journal Article
Exposure Or Hazard Target
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International Journal of Molecular Sciences, 2013, 14(8): 15546-15560
International Journal of Molecular Sciences
Watanabe M, Yoneda M, Morohashi A, Hori Y, Okamoto D, Sato A, Kurioka D, Nittami T, Hirokawa Y, Shiraishi T, Kawai K, Kasai H, Totsuka Y
Last updated on August 21, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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