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Characterization of carbon nanotube protein corona by using quantitative proteomics
Link to Journal Abstract
The protein corona of a nanomaterial is a complex layer of proteins spontaneously and stably formed when the material is exposed to body fluids or intracellular environments. In this study, we utilised stable isotope labeling by amino acids in cell culture (SILAC)-based quantitative proteomics to characterise the binding of human cellular proteins to two forms of carbon nanoparticles: namely multi-walled carbon nanotubes (MWCNTs) and carbon black (CB). The relative binding efficiency of over 750 proteins to these materials is measured. The data indicate that MWCNTs and CB bind to vastly different sets of proteins. The molecular basis of selectivity in protein binding is investigated. This study is the first large-scale characterisation of protein corona on CNT, providing the biochemical basis for the assessment of the suitability of CNTs as biomedical tools, and as an emerging pollutant.
This study is the first large-scale characterisation of protein corona on carbon nanotubes (CNT), providing the biochemical basis for the assessment of the suitability of CNTs as biomedical tools, and as an emerging pollutant.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Material Analysis and Applications
Risk Exposure Group
Nanomedicine: Nanotechnology, Biology and Medicine, 9(5): 583-593 (July 2013)
Nanomedicine: Nanotechnology, Biology and Medicine
Cai X, Ramalingam R, Wong HS, Cheng J, Ajuh P, Cheng SH, Lam YW
Last updated on August 9, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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