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Title: Effect of Biologically Synthesized Silver Nanoparticles on Human Cancer Cells
Date: 12/2012
  Link to Journal Abstract
Abstract: Nanotechnology as a field of knowledge is burgeoning day by day, making an impact in all spheres of human life. Biological methods of synthesis have paved way for the ¡°greener synthesis¡° of nanoparticles. The ¡°green synthesis¡° has proven to be a better method due to their slower kinetics, better manipulation, control over crystal growth and stabilization. Recent advancement in the field includes enzymatic method of synthesis suggesting the enzymes to be responsible for the nanoparticle formation. The biomedical applications of silver nanoparticle (AgNPs) can be effective by the use of biologically synthesized nanoparticles which minimize the factors like cost of synthesis and toxicity of product. These are further found to be exceptionally stable when compared with the nanoparticles prepared through other methods. In the present study, silver nanoparticles were biologically synthesized using pure enzyme ¦Á-amylase and the other one by using soluble proteins of neem leaf extracts. The nanoparticles were characterized by UV-Visible absorption, Transmission Electron Microscopy (TEM) and X-ray Diffraction (XRD). In addition to the normal cell line (INT-407), the cytotoxicity of the prepared nanoparticles was evaluated on human cervical cancer cells (SiHa) as well. The results indicated that the IC50 of neem extracted AgNPs and ¦Á-amylase were at marginally variable concentrations that is; lower concentration of ¡Ü4.25 ¦Ìg/ml for the former and ¡Ü32.5 ¦Ìg/ml for the latter. In addition, our results also demonstrated that in vitro cytotoxicity assessment of the AgNPs has significant correlation with the total protein concentration in treated cells. These findings confirm the cytotoxic properties of AgNPs, and suggest that they may be cost-effective and an alternative measure in the field of cancer therapeutics.
Non-technical Summary: In the present study, silver nanoparticles were biologically synthesized using pure enzyme ¦Á-amylase and the other one by using soluble proteins of neem leaf extracts. The cytotoxicity of the prepared nanoparticles was evaluated on the normal cell line (INT-407) as well as human cervical cancer cells (SiHa).
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Mammalian
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Metal
Production Method Engineered
Risk Exposure Group General Population
Target Audience Technical Research
Citation: Science of Advanced Materials, 4(12): 1200-1206 (December 2012)
Publication: Science of Advanced Materials
Author: Mishra A, Mehdi SJ, Irshad MD, Ali A, Sardar M, Moshahid M, Rizvi A
Volume: 4
Number: 12
Pages: 1200-1206
     


Last updated on March 11, 2013
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
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