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Cytotoxicity and Genotoxicity of Ceria Nanoparticles on Different Cell Lines in Vitro
Link to Journal Abstract
Owing to their radical scavenging and UV-filtering properties, ceria nanoparticles (CeO2-NPs) are currently used for various applications, including as catalysts in diesel particulate filters. Because of their ability to filter UV light, CeO2-NPs have garnered significant interest in the medical field and, consequently, are poised for use in various applications. The aim of this work was to investigate the effects of short-term (24 h) and long-term (10 days) CeO2-NP exposure to A549, CaCo2 and HepG2 cell lines. Cytotoxicity assays tested CeO2-NPs over a concentration range of 0.5 ėg/mL to 5000 ėg/mL, whereas genotoxicity assays tested CeO2-NPs over a concentration range of 0.5 ėg/mL to 5000 ėg/mL. In vitro assays showed almost no short-term exposure toxicity on any of the tested cell lines. Conversely, long-term CeO2-NP exposure proved toxic for all tested cell lines. NP genotoxicity was detectable even at 24-h exposure. HepG2 was the most sensitive cell line overall; however, the A549 line was most sensitive to the lowest concentration tested. Moreover, the results confirmed the ceria nanoparticles capacity to protect cells when they are exposed to well-known oxidants such as H2O2. A Comet assay was performed in the presence of both H2O2 and CeO2-NPs. When hydrogen peroxide was maintained at 25 ėM, NPs at 0.5 ėg/mL, 50 ėg/mL, and 500 ėg/mL protected the cells from oxidative damage. Thus, the NPs prevented H2O2-induced genotoxic damage.
The aim of this work was to investigate the effects of short-term (24 h) and long-term (10 days) ceria nanoparticle (CeO2-NP) exposure to A549, CaCo2 and HepG2 cell lines.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
International Journal of Molecular Sciences, 2013, 14(2): 3065-3077
International Journal of Molecular Sciences
De Marzi L, Monaco A, De Lapuente J, Ramos D, Borras M, Di Gioacchino M, Santucci S, Poma A
Last updated on February 27, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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