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Title:

Microfluidic Image Cytometry (micro-FIC) Assessments of Silver Nanoparticle Cytotoxicity

Date:

12/2012

Link to Journal Abstract

Abstract:

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry (micro-FIC). The LC50 value of HeLa cells exposed to AgNPs in the microfluidic device was estimated as 46.7 mg/L, which is similar to that estimated by MTT-based IC for cells cultured in a 96 well plate (49.9 mg/L). These results confirm that the micro-FIC approach can produce cytotoxicity data that is reasonably well-matched with that of the conventional 96 well plate system with much higher efficiency. This micro-FIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs. This may aid in speeding up data acquisition in the field of nanosafety and make a significant contribution to the quantitative understanding of nanoproperty-toxicity relationships.

Non-technical Summary:

Cytotoxicity assessment of silver nanoparticles (AgNPs) was performed using MTT-based microfluidic image cytometry. This micro-FIC method provides many benefits including ease of use and low cost, and is a more rapid in vitro cell based assay for AgNPs.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Mammalian

Exposure Pathway

Other/Unspecified

Method Of Study

Material Analysis and Applications

In Vitro

Paper Type

Hazard

Applications