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Zinc oxide nanoparticles and monocytes: Impact of size, charge and solubility on activation status
Link to Journal Abstract
Zinc oxide (ZnO) particle induced cytotoxicity was dependent on size, charge and solubility, factors which at sublethal concentrations may influence the activation of the human monocytic cell line THP1. ZnO nanoparticles (NP; average diameter 70 nm) were more toxic than the bulk form (< 44 ìm mesh) and a positive charge enhanced cytotoxicity of the NP despite their relatively high dissolution. A positive charge of the particles has been shown in other studies to have an influence on cell viability. Centrifugal filtration using a cut off of 5 kDa and Zn element analysis by atomic absorption spectroscopy confirmed that exposure of the ZnO particles and NP to 10% foetal bovine serum resulted in a strong association of the Zn2 + ion with protein. This association with protein may influence interaction of the ZnO particles and NP with THP1 cells. After 24 h exposure to the ZnO particles and NP at sublethal concentrations there was little effect on immunological markers of inflammation such as HLA DR and CD14, although they may induce a modest increase in the adhesion molecule CD11b. The cytokine TNFá is normally associated with proinflammatory immune responses but was not induced by the ZnO particles and NP. There was also no effect on LPS stimulated TNFá production. These results suggest that ZnO particles and NP do not have a classical proinflammatory effect on THP1 cells.
For this study, the authors investigated the effects of manufactured zinc oxide nanoparticles (ZnO NPs) on a model of innate immune cell response, the human monocytic cell line THP1. ZnO NPs were provided as anionic, cationic and non-ionic colloidal dispersions allowing the study to address the effect of charge on the monocyte response.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Toxicology and Applied Pharmacology, 266(1): 19-26 (January 2013)
Toxicology and Applied Pharmacology
Prach M, Stone V, Proudfoot L
Last updated on February 13, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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