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Title: Evaluating cell specific cytotoxicity of differentially charged silver nanoparticles
Date: 1/2013
  Link to Journal Abstract
Abstract: Silver nanoparticles (AgNPs) are one of the most commercially viable nanotechnological products, nevertheless; safety issues are raised regarding the use of such nanoparticles due to unintentional health and environmental impacts. In the present study, AgNPs were synthesized by chemically reducing silver nitrate alternatively with sodium borohydride, tannic acid, ascorbic acid and sodium citrate. AgNPs synthesized by reduction with tannic acid (TSNPs) and sodium borohydride (BSNPs) exhibited highest and lowest surface potential respectively. Therefore these two types of AgNPs were selected for their toxicity assessment in cellular environment. We treated skin epithelial A431, lung epithelial A549 and murine macrophages RAW264.7 cells with AgNPs over a range of doses (5–100 ìg/ml). Toxicity was evaluated by measuring changes in cellular morphology, ROS generation, metabolic activity and expression of various stress markers. Interestingly, TSNPs exhibited a higher negative zeta-potential and also higher toxicity. Higher toxicity of TSNPs was attested by dose-dependent increase in cellular disruption and ROS generation. BSNPs showed cytotoxic effect up to the concentration of 50 ìg/ml and thereafter the cytotoxic effect attenuated. TSNPs induced a dose dependent increase in the expression of stress markers pp38, TNF-á and HSP-70. Our report proposes that cytotoxicity of AgNPs changes with surface potential of nanoparticles and cells type.
Non-technical Summary: In this study, silver nanoparticles (AgNPs) were synthesized by chemically reducing silver nitrate alternatively with sodium borohydride, tannic acid, ascorbic acid and sodium citrate. Then skin epithelial A431, lung epithelial A549 and murine macrophages RAW264.7 cells were treated with the prepared AgNPs over a range of doses. Toxicity was evaluated by measuring changes in cellular morphology, ROS generation, metabolic activity and expression of various stress markers.
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Mammalian
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Metal
Production Method Engineered
Risk Exposure Group General Population
Target Audience Technical Research
Citation: Food and Chemical Toxicology, 51: 1-14 (January 2013)
Publication: Food and Chemical Toxicology
Author: Kaur J, Tikoo K
Volume: 51
Pages: 1-14
     


Last updated on February 11, 2013
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
Rice University