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Stability of Nanoparticle Suspensions in Different Biologically Relevant Media
Link to Journal Paper
For application of nanoparticles (NPs) as delivery vectors for biomedical applications the size, charge, surface chemistry and aggregation of the particles are one of key parameters. We focused on characterization of maghemite and cobalt ferrite magnetic NPs by measuring size distribution and zeta potential in different culture media and PBS. We show that our NPs functionalized with PAA are relatively very stable also in different culture media, where level of aggregation depends on medium composition. Effect of divalent ions and serum presence on stability is specifically analysed and we present possible destabilization mechanisms. We show that stability of electrostatically stabilized suspensions is affected by the molar concentration and valence of destabilizing counterions like Na+ or Mg2+ where effective surface charge of nanoparticles and thus repulsion force is screened by these counterions. In agreement with other papers we demonstrate significant effect of media composition (divalent ions, serum) and thus show the importance of NPs characterization under conditions that are representative of cell culture media or physiological conditions for understanding of NPs interaction with biological systems and for assessments of nanoparticle cytotoxicity. Further, by understanding the destabilization mechanisms one can anticipate effect in different media and to some degree predict behaviour of nanoparticle suspensions.
This study focuses on characterization of maghemite and cobalt ferrite magnetic nanoparticles (NPs) by measuring size distribution and zeta potential in different culture media and PBS. The effect of media composition (divalent ions, serum), which can show the importance of NPs characterization under conditions that are representative of cell culture media or physiological conditions for understanding of NPs interaction with biological systems and for assessments of nanoparticle cytotoxicity was also evaluated.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Material Analysis and Applications
Risk Exposure Group
Digest Journal of Nanomaterials and Biostructures, 7(4): 1389-1400 (October-December 2012)
Digest Journal of Nanomaterials & Biostructures
Pavlin M, Bregar VB
Last updated on January 30, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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