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Title: Size-dependent toxicity and cell interaction mechanisms of gold nanoparticles on mouse fibroblasts
Date: 3/2013
  Link to Journal Abstract
Abstract: Gold nanoparticles (AuNPs) are currently used in several fields including biomedical applications, although no conclusive information on their cytotoxicity is available. For this reason this work has investigated the effects of AuNPs in vitro on Balb/3T3 mouse fibroblasts. Results obtained exposing cells for 72 h to AuNPs 5 and 15 nm citrate stabilized, revealed cytotoxic effects only for AuNPs 5 nm at concentration ¡Ý 50 ¦ÌM if measured by colony forming efficiency (CFE). To understand the differences in cytotoxicity observed for the two AuNPs sizes, we investigated the uptake and the intracellular distribution of the nanoparticles. By TEM it was observed that 5 and 15 nm AuNPs are internalized by Balb/3T3 cells and located within intracellular endosomal compartments. Quantification of the uptake by ICP-MS showed that AuNPs internalization enhanced even up to 72 h. Disruption of the actin cytoskeleton was evident, with cell footprints narrow and contracted; effects more remarkable in cells exposed to 5 nm AuNP. The mechanism of NPs cell internalization was investigated using immunocytochemistry and western blot. No significant effect was observed in the expression level of caveolin, while reduction of the expression and degradation of the clathrin heavy chain was observed in cells exposed for 72 h to AuNPs.
Non-technical Summary: This work investigates the effects of gold nanoparticles (AuNPs) in vitro on Balb/3T3 mouse fibroblasts. Results were obtained by exposing cells for 72 h to AuNPs 5 and 15 nm, citrate stabilized. To understand the differences in cytotoxicity observed for the two AuNPs sizes, the authors investigated the uptake and the intracellular distribution of the nanoparticles.
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Mammalian
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Metal
Production Method Engineered
Risk Exposure Group General Population
Target Audience Technical Research
Citation: Toxicology Letters, 217(3): 215-216 (March 2013)
Publication: Toxicology Letters
Author: Coradeghini R, Gioria S, Garcia CP, Nativo P, Franchini F, Gilliland D, Ponti J, Rossi F
Volume: 217
Number: 3
Pages: 205-216
     


Last updated on February 11, 2013
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
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