ICON Web & News
Search Using OECD Database
Return to Previous Page
Addition or Correction
Size-dependent toxicity and cell interaction mechanisms of gold nanoparticles on mouse fibroblasts
Link to Journal Abstract
Gold nanoparticles (AuNPs) are currently used in several fields including biomedical applications, although no conclusive information on their cytotoxicity is available. For this reason this work has investigated the effects of AuNPs in vitro on Balb/3T3 mouse fibroblasts. Results obtained exposing cells for 72 h to AuNPs 5 and 15 nm citrate stabilized, revealed cytotoxic effects only for AuNPs 5 nm at concentration ¡Ý 50 ¦ÌM if measured by colony forming efficiency (CFE). To understand the differences in cytotoxicity observed for the two AuNPs sizes, we investigated the uptake and the intracellular distribution of the nanoparticles. By TEM it was observed that 5 and 15 nm AuNPs are internalized by Balb/3T3 cells and located within intracellular endosomal compartments. Quantification of the uptake by ICP-MS showed that AuNPs internalization enhanced even up to 72 h. Disruption of the actin cytoskeleton was evident, with cell footprints narrow and contracted; effects more remarkable in cells exposed to 5 nm AuNP. The mechanism of NPs cell internalization was investigated using immunocytochemistry and western blot. No significant effect was observed in the expression level of caveolin, while reduction of the expression and degradation of the clathrin heavy chain was observed in cells exposed for 72 h to AuNPs.
This work investigates the effects of gold nanoparticles (AuNPs) in vitro on Balb/3T3 mouse fibroblasts. Results were obtained by exposing cells for 72 h to AuNPs 5 and 15 nm, citrate stabilized. To understand the differences in cytotoxicity observed for the two AuNPs sizes, the authors investigated the uptake and the intracellular distribution of the nanoparticles.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Toxicology Letters, 217(3): 215-216 (March 2013)
Coradeghini R, Gioria S, Garcia CP, Nativo P, Franchini F, Gilliland D, Ponti J, Rossi F
Last updated on February 11, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
Why Join Us?
Mission and Strategy
Good Nano Guide
Nano EHS Research Needs
Current Practices Survey