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Spatiotemporal Visualization of Subcellular Dynamics of Carbon Nanotubes
Link to Journal Abstract
To date, there is no consensus on the relationship between the physicochemical characteristics of carbon nanotubes (CNTs) and their biological behavior; however, there is growing evidence that the versatile characteristics make their biological fate largely unpredictable and remain an issue of limited knowledge. Here we introduce an experimental methodology for tracking and visualization of postuptake behavior and the intracellular fate of CNTs based on the spatial distribution of diffusion values throughout the plant cell. By using raster scan image correlation spectroscopy (RICS), we were able to generate highly quantitative spatial maps of CNTs diffusion in different cell compartments. The spatial map of diffusion values revealed that the uptake of CNTs is associated with important subcellular events such as carrier-mediated vacuolar transport and autophagy. These results show that RICS is a useful methodology to elucidate the intracellular behavior mechanisms of carbon nanotubes and potentially other fluorescently labeled nanoparticles, which is of relevance for the important issues related to the environmental impact and health hazards.
This paper introduces an experimental methodology for tracking and visualization of postuptake behavior and the intracellular fate of carbon nanotubes (CNTs) based on the spatial distribution of diffusion values throughout the plant cell, using raster scan image correlation spectroscopy (RICS).
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Material Analysis and Applications
Risk Exposure Group
Nano Letters, 2012, 12(12): 6145-6151
Serag MF, Braeckmans K, Habuchi S, Kaji N, Bianco A, Baba Y
Last updated on January 18, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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