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Title: Effect of Al2O3 nanoparticles on bacterial membrane amphiphilic biomolecules
Date: 2/2013
  Link to Journal Abstract
Abstract: Lipopolysaccharide (LPS) and l-á-phosphatidyl-ethanolamine (PE) are amphiphilic biomolecules that are key constituents in the outer cell membranes of Gram-negative bacteria. In this study, micelles and vesicles of LPS/PE were used as cell membrane models to evaluate nanoparticle (NP) effects on membrane structure. Using atomic force microscopy and sorption experiments, we observed that LPS vesicles were dispersed by Al2O3 NPs because of the strong attachment between LPS polysaccharide chain and oxide surface. LPS coated on the Al2O3 NPs, and formed a layer of tens of nanometer as shown from atom force microscopy (AFM) images. High pH, ionic strength and sulfate concentration inhibited the adsorption of LPS molecules on NPs. The features of PE vesicles were changed after exposing to Al2O3 NPs, inducing large round vesicles with detectable thickness as revealed by AFM, resulting in the increase of vesicle diameter. The aqueous stability of PE was disturbed when adding Al2O3 NPs, but was enhanced by increasing pH. The interaction between NPs and membrane amphiphilic biomolecules may affect membrane fluidity, integrity and lateral organization, which is important for NP safety evaluation and for new NP designs in biological and biomedical applications.
Non-technical Summary: Lipopolysaccharide (LPS) and l-alpha-phosphatidyl-ethanolamine (PE) are amphiphilic biomolecules that are key constituents in the outer cell membranes of Gram-negative bacteria. In this study, micelles and vesicles of LPS/PE were used as cell membrane models to evaluate nanoparticle (NP) effects on membrane structure.
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Other/Unspecified
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Oxide
Production Method Engineered
Risk Exposure Group Other/Unspecified
Target Audience Technical Research
Citation: Colloids and Surfaces B: Biointerfaces, 102: 292-299 (February 2013)
Publication: Colloids and Surfaces B: Biointerfaces
Author: Jiang W, Ghosh S, Song L, Vachet RW, Xing B
Volume: 102
Pages: 292-299
     


Last updated on January 9, 2013
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
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