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Multi walled carbon nano tubes induced hepatotoxicity in Swiss albino mice
Link to Journal Abstract
In the present study, multi walled carbon nano tubes (MWCNTs) were synthesized using chemical vapour deposition (CVD) technique. Swiss albino mice were orally administered with single dose of 60 and 100 mg/kg body weight of purified and functionalized MWCNTs suspended in water. The mice were autopsied on 7, 14, 21 and 28 days post exposure. Liver was taken out and part of it fixed in Bouin's solution for histopathological examinations. The remaining part was immersed in cold saline, blotted dry, weighed quickly and homogenized in ice cold buffer. The activity of superoxide dismutase (SOD) and catalase (CAT) was immediately measured in the supernatant. The MWCNTs in liver led to pathological changes, including injury to macrophages, cellular swelling, unspecific inflammation, spot necrosis and blood coagulation. Estimation of SOD and CAT showed altered levels in the experimental groups as compared to controls. Therefore, MWCNTs from manufactured and combustion sources in the environment can have adverse effects on human health.
In this study, multi walled carbon nano tubes (MWCNTs) were synthesized using chemical vapour deposition (CVD) technique and swiss albino mice were orally administered with single dose of 60 and 100 mg/kg body weight of purified and functionalized MWCNTs suspended in water. The mice were autopsied on several days post exposure. Liver was taken out and part of it fixed in Bouin's solution for histopathological examinations.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Micron, 44: 359-364 (January 2013)
Awasthi KK, John PJ, Awasthi A, Awasthi K
Last updated on January 3, 2013
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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