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Effects of Iron Oxide Nanoparticle Labeling on Human Endothelial Cells
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Iron oxide nanoparticles (INOPS) are a potential contrast agent for magnetic resonance (MR) tracking of transplanted endothelial cells. The objective of this study was to examine the effect of INOPS labeling on endothelial cells. The mixture of INOPS and poly-l-lysine (PLL) was used to label human endothelial cells. Labeling efficiency was examined by Prussian blue staining, transmission electron microscopy, and atomic absorption spectrometry. The effect of iron oxide concentration on cell viability and proliferation were determined. The correlation of reactive oxygen species (ROS) and apoptosis was also examined. In vitro MRI scanning was carried out using a 1.5T MR system. INOPS-PLL could be readily taken up by endothelial cells and subsequently induce MRI signal intensity changes. However, higher labeling concentration (>50 µg/ml) and longer incubation (48 h) can affect cell viability and proliferation. Mitochondrial damage, apoptosis, and autolysosmes were observed under high INOPS-PLL concentrations, which were correlated to ROS production. INOPS-PLL nanoparticles can be used to label transplanted endothelial cells. However, high concentration of INOPS can impair cell viability, possibly through ROS-mediated apoptosis and autophagy.
The objective of this study was to examine the effect of iron oxide nanoparticles (INOPS) labeling on endothelial cells. The effect of iron oxide concentration on cell viability and proliferation were determined and the correlation of reactive oxygen species (ROS) and apoptosis was also examined.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
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Cell Transplantation, 2012, 21(9): 1805-1820
Yang FY, Yu MX, Zhou Q, Chen WL, Gao P, Huang Z
Last updated on December 21, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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