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Toxicity of Aqueous Fullerene nC60 to Activated Sludge: Nitrification Inhibition and Microtox Test
Link to Journal Abstract
The increasing production and use of fullerene nanomaterials raised their exposure potential to the activated sludge during biological wastewater treatment process. In this study, the toxicity of aqueous nanoscaled C60 (nC60) to activated sludge was investigated using nitrification inhibition and Microtox test. The test solutions of nC60 were prepared using two methods: long stirring (Stir/nC60) and toluene exchange (Tol/nC60). The nC60 aggregation in test medium was also evaluated for toxicity assessment. The results showed that the nC60 aggregation behaved differently in two test mediums during the incubation periods. The nC60 toxicity was greatly influenced by the preparation method. Stir/nC60 presented no significant toxicity to both the nitrification sludge and bioluminescent bacteria at the maximum concentration studied. In contrast, the EC20 of Tol/nC60 was obtained to be 4.89?mg/L (3?h) for the nitrification inhibition and 3.44?mg/L (30?min) for Microtox test, respectively.
In this study, the toxicity of aqueous nanoscaled C60 (nC60) to activated sludge was investigated using nitrification inhibition and Microtox test. The test solutions of nC60 were prepared using two methods: long stirring (Stir/nC60) and toluene exchange (Tol/nC60). The nC60 aggregation in test medium was also evaluated for toxicity assessment.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Environmental Fate and Transport
Risk Exposure Group
Journal of Nanomaterials, 2012, Article ID 512956 (6 pp)
Journal of Nanomaterials
Yang Y, Nakada N, Nakajima R, Wang C, Tanaka H
Article ID 512956
Last updated on October 12, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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