International Council on Nanotechnology

ICON Web & News Virtual Journal Everything

Resources
	Virtual Journal
	Analyze Database
	GoodNanoGuide
	ICON Reports
	ICON Backgrounders
	Glossary
	Policy Reports
	Ratings Comment Guidelines
	Links

Quick Search:
Keywords: Search: Search Using OECD Database

Details

Return to Previous Page

Addition or Correction

Title:

Cytotoxicity of Carbon Nanotubes on J774 Macrophages Is a Purification-Dependent Effect

Date:

5/2012

Link to Journal Abstract

Abstract:

The cytotoxicity of the carbon nanotubes (CNTs) is an important factor for the manufacture of nanovaccines. The aim of this work was to evaluate the relationship of the purification method of CNTs in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24?h of exposure at concentrations of 0.06, 0.6, and 6?mg/L of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3 and (2) sonication in H2SO4/HNO3. Characterization and COOH content of CNTs was performed using scanning electron microscopy, raman spectroscopy, and titration with NaHCO3. P-CNTs1 had lengths >100??m and 2.76% COOH content, while P-CNTs2 had lengths >1??m and 7% COOH content. This last particle showed a lower toxic effect. The results suggest that the lenght and COOH content are important factors in the toxicity of the CNTs.

Non-technical Summary:

The aim of this work was to evaluate the relationship of the purification method of carbon nanotubes (CNTs) in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24?h of exposure at various concentrations of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3 and (2) sonication in H2SO4/HNO3.

Content Emphasis

Peer Reviewed Journal Article

Exposure Or Hazard Target

Exposure Pathway

Method Of Study

Paper Type

Particle Type