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Cytotoxicity of Carbon Nanotubes on J774 Macrophages Is a Purification-Dependent Effect
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The cytotoxicity of the carbon nanotubes (CNTs) is an important factor for the manufacture of nanovaccines. The aim of this work was to evaluate the relationship of the purification method of CNTs in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24?h of exposure at concentrations of 0.06, 0.6, and 6?mg/L of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3 and (2) sonication in H2SO4/HNO3. Characterization and COOH content of CNTs was performed using scanning electron microscopy, raman spectroscopy, and titration with NaHCO3. P-CNTs1 had lengths >100??m and 2.76% COOH content, while P-CNTs2 had lengths >1??m and 7% COOH content. This last particle showed a lower toxic effect. The results suggest that the lenght and COOH content are important factors in the toxicity of the CNTs.
The aim of this work was to evaluate the relationship of the purification method of carbon nanotubes (CNTs) in cellular toxicity using macrophages (MOs) from the J774 cell line. Viability test was performed with MTT assays at 24?h of exposure at various concentrations of unpurified (UP-CNTs) or purified (P-CNTs) CNTs by two different methods: (1) reflux with 3M HNO3 and (2) sonication in H2SO4/HNO3.
Peer Reviewed Journal Article
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Method Of Study
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Journal of Nanomaterials, 2012, Article ID 715838 (7 pp)
Journal of Nanomaterials
Montes-Fonseca SL, Orrantia-Borunda E, Duarte-Moller A, Luna-Velasco A, Roman-Aguirre M, Horta CG, Sanchez-Ramirez B
Article ID 715838
Last updated on October 12, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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