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Induction of Apoptosis in Human Endothelial Cells by Nanodiamond Particles
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Carbon nanoparticles are a promising material which finds application in different fields in industry and medicine. For medical applications, biocompatibility of nanoparticles is of critical importance because a lot of medical implants are coated by carbon coating. Our previous results showed that nanoparticles may induce increased production of ROS by the cells so we decided to checked if nanopowders can induce apoptosis. Apoptosis was quantified by double-staining with acridine orange and ethidium bromide. For comparison, we identified apoptotic cells with annexin V-FITC/propidium iodide. Our data demonstrate that treatment of the cells with diamond nanopowders may induce apoptosis and necrosis and this effect is dependent on the time of treatment and concentration of the nanopowders. The highest level of apoptotic cells was observed after incubation with Ultrananocrystalline Detonation Diamond (UDD) suggesting that the size is the main determinant of nanoparticle cytotoxicity.
This study investigated whether diamond nanopowders can induce cell apoptosis. Apoptosis was quantified by double-staining with acridine orange and ethidium bromide. For comparison, the authors identified apoptotic cells with annexin V-FITC/propidium iodide.
Peer Reviewed Journal Article
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Journal of Nanoscience and Nanotechnology, 12(6): 5117-5121 (June 2012)
Journal of Nanoscience and Nanotechnology
Solarska K, Gajewska A, Bartosz G, Mitura K
Last updated on September 11, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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