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Title: Cytotoxicity of functionalized carbon nanotubes in J774A macrophages
Date: 8/2012
  Link to Journal Abstract
Abstract: Cytotoxicity of carbon nanotubes (CNTs) is a prime concern for its use as antigen carriers. Here we evaluated the cytotoxic effect of unpurified (UP-CNTs), purified (P-CNTs), fluorescein isothiocyanate–functionalized (FITC-CNTs), and Entamoeba histolytica 220-kDa lectin–functionalized CNTs (L220-CNTs) in J774A macrophage (MOs) cell line. Cell viability and apoptosis were analyzed by MTT and TUNEL assays, respectively. Cyclooxygenase-2 (COX-2) was analyzed by reverse transcription–polymerase chain reaction. Cytotoxicity at 6.0 mg/L was higher with UP-CNTs > P-CNTs > FITC-CNTs, showing a decrease in cell viability and an increase in apoptosis. In contrast, MOs interacted with L220-CNTs showed an increase in cell viability without signs of apoptosis. Although UP-CNTs and P-CNTs exhibited COX-2 induction with 6.0 mg/L, functionalized CNTs were able to induce COX-2 at concentrations as low as 0.06 mg/L. These results suggest that functionalization decreases toxicity, and that L220-CNTs may be an excellent candidate for the production of a nanovaccine against amebiasis.
Non-technical Summary: This study evaluates the cytotoxic effect of unpurified carbon nanotubesd (UP-CNTs), purified (P-CNTs), fluorescein isothiocyanate–functionalized (FITC-CNTs), and Entamoeba histolytica 220-kDa lectin–functionalized CNTs (L220-CNTs) in J774A macrophage (MOs) cell line. Cell viability and apoptosis were analyzed by MTT and TUNEL assays, respectively.
Content Emphasis Peer Reviewed Journal Article
Exposure Or Hazard Target Mammalian
Exposure Pathway Other/Unspecified
Method Of Study In Vitro
Paper Type Hazard
Particle Type Carbon
Production Method Engineered
Risk Exposure Group General Population
Target Audience Technical Research
Citation: Nanomedicine: Nanotechnology, Biology and Medicine, 8(6): 853-859 (August 2012)
Publication: Nanomedicine: Nanotechnology, Biology and Medicine
Author: Montes-Fonseca SL, Orrantia-Borunda E, Aguilar-Elguezabal A, Horta CG, Talamas-Rohana P, Sanchez-Ramirez B
Volume: 8
Number: 6
Pages: 853-859
     


Last updated on October 12, 2012
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This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.

 		 
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