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Cytotoxicity of functionalized carbon nanotubes in J774A macrophages
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Cytotoxicity of carbon nanotubes (CNTs) is a prime concern for its use as antigen carriers. Here we evaluated the cytotoxic effect of unpurified (UP-CNTs), purified (P-CNTs), fluorescein isothiocyanate–functionalized (FITC-CNTs), and Entamoeba histolytica 220-kDa lectin–functionalized CNTs (L220-CNTs) in J774A macrophage (MOs) cell line. Cell viability and apoptosis were analyzed by MTT and TUNEL assays, respectively. Cyclooxygenase-2 (COX-2) was analyzed by reverse transcription–polymerase chain reaction. Cytotoxicity at 6.0 mg/L was higher with UP-CNTs > P-CNTs > FITC-CNTs, showing a decrease in cell viability and an increase in apoptosis. In contrast, MOs interacted with L220-CNTs showed an increase in cell viability without signs of apoptosis. Although UP-CNTs and P-CNTs exhibited COX-2 induction with 6.0 mg/L, functionalized CNTs were able to induce COX-2 at concentrations as low as 0.06 mg/L. These results suggest that functionalization decreases toxicity, and that L220-CNTs may be an excellent candidate for the production of a nanovaccine against amebiasis.
This study evaluates the cytotoxic effect of unpurified carbon nanotubesd (UP-CNTs), purified (P-CNTs), fluorescein isothiocyanate–functionalized (FITC-CNTs), and Entamoeba histolytica 220-kDa lectin–functionalized CNTs (L220-CNTs) in J774A macrophage (MOs) cell line. Cell viability and apoptosis were analyzed by MTT and TUNEL assays, respectively.
Peer Reviewed Journal Article
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Nanomedicine: Nanotechnology, Biology and Medicine, 8(6): 853-859 (August 2012)
Nanomedicine: Nanotechnology, Biology and Medicine
Montes-Fonseca SL, Orrantia-Borunda E, Aguilar-Elguezabal A, Horta CG, Talamas-Rohana P, Sanchez-Ramirez B
Last updated on October 12, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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