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Evaluation on Cytotoxicity and Genotoxicity of the L-Glutamic Acid Coated Iron Oxide Nanoparticles
Link to Journal Abstract
To evaluate the cytotoxicity and genotoxicity of L-glutamic acid (Glu) coated Fe2O3 nanoparticles (hereafter refer as Glu@MNPs) on Chinese Hamster Lung (CHL) cells using Trypan blue dye exclusion assay, Oxidative stress markers, Comet assay and micronucleus (MN) assay. Results showed a low cytotoxicity with an IC50 was 254.739 ėg/ml 36 h post incubation period in CHL cells. Furthermore, Cell redox status is slightly disturbed: Glu@MNPs exposure cause reactive oxygen species production, glutathione depletion and inactivation of some antioxidant enzymes: glutathione reduc-tase, superoxide dismutase, but not catalase. Moreover, no significant genotoxic response was observed in CHL cells over concentration ranges from 8 to 128 ėg/mL for all exposure time periods. The results suggest that the Glu@MNPs show biocompatibility In Vitro.
This study was undertaken to evaluate the cytotoxicity and genotoxicity of L-glutamic acid (Glu) coated Fe2O3 nanoparticles on Chinese Hamster Lung (CHL) cells using Trypan blue dye exclusion assay, Oxidative stress markers, Comet assay and micronucleus (MN) assay.
Peer Reviewed Journal Article
Exposure Or Hazard Target
Method Of Study
Risk Exposure Group
Journal of Nanoscience and Nanotechnology, 12(3): 2866-2873 (March 2012)
Journal of Nanoscience and Nanotechnology
Zhang T, Qian L, Tang M, Xue Y, Kong L, Zhang S, Pu Y
Last updated on July 12, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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