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Cytotoxicity and TNF-alpha Secretion in RAW264.7 Macrophages Exposed to Different Fullerene Derivatives
Link to Journal Abstract
Fullerene derivatives have been reported as potential nanomedicines, however the role of surface chemical modification on the biological effects remains unclear. In this study five kinds of water soluble C60 derivatives with different surface chemical modification, C60-(OH)20 (HFD), C60-(â-Ala)10.1 (AFD), C60-(Lys)8.7 (KFD), C60-(Arg)8.6 (RFD) and C60-(NH(CH2)2NH2)8.8 (NFD) were synthesized. Their cytotoxicity as well as TNF-á secretions were evaluated in RAW264.7 macrophage cell line. The results show that no significant cytotoxicity can be observed upon 24 h exposure to C60 derivatives at less than 50 ìg/mL However, higher concentration (>100 ìg/mL) of these C60 derivatives decreases the proliferation of RAW264.7. The cytotoxicity of these fullerene derivatives is probably through the apoptosis pathway, while the extent of cytotoxicity varies with the different surface charges. Higher celluar uptake of HFD was observed in RAW264.7 cells than AFD, which correlates with the more toxic effect of HFD over AFD. The secretion of cytokine tumor necrosis factor alpha (TNF-á) was determined to evaluate the immunostimulating activity of these fullerene derivatives. The data show that the fullerene derivatives with negative surface charges secrete more TNF-alpha, whereas derivatives with positive charges show insignificant effect. The possible influence of various surface charge property on the observed biological effects is discussed.
In this study, five kinds of water soluble C60 derivatives with different surface chemical modification were synthesized and their cytotoxicity as well as TNF-á secretions were evaluated in RAW264.7 macrophage cell line.
Peer Reviewed Journal Article
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Journal of Nanoscience and Nanotechnology, 12(3): 2169-2178 (March 2012)
Journal of Nanoscience and Nanotechnology
Xiang K, Dou Z, Li Y, Xu Y, Zhu J, Yang S, Sun H, Liu Y
Last updated on July 10, 2012
This work is supported in part by the Nanoscale Science and Engineering Initiative of the National Science Foundation
under NSF Award Number EEC-0118007.
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